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In the second incubation step
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian IFNa
You can calculate the concentration of Mouse αFP in the samples by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Mouse SPP1
Human RELN (Reelin) ELISA Kit Packaging:96 wells In the second incubation stepThe Human (RELN) Reelin ELISA Kit measures Reelin in samples. The plate has been pre coated with Human RELN antibody. RELN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human RELN Antibody is added and binds to RELN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated RELN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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